International Journal of Trend in Scientific Research and Development: Biochemistry

International Journal of Trend in Scientific Research and Development: Biochemistry

Showing posts with label Biochemistry. Show all posts

Showing posts with label Biochemistry. Show all posts

Saturday 9 October 2021

Oxidative Stress in Diabetic Obese Patients Attending Selected Tertiary Hospital in Abia State Nigeria

Team IJTSRD October 09, 2021 0

Oxidative Stress in Diabetic Obese Patients Attending Selected Tertiary Hospital in Abia State Nigeria

Oxidative stress has continued to play major roles in the etiology of many metabolic diseases, diabetes and obesity inclusive. The study on oxidative stress in diabetic obese patients was carried out with patients from two tertiary hospitals in Abia State Nigeria who gave their consent to participate in the study. A total of 120 patients 18 years and above who were stratified into five different age groups were enrolled for the study. Following approved protocols fasting blood samples were collected and a semi structured questionnaire used to collect other data needed for the study. Data was analyzed using T test and SPSS version 20.0 software and result considered significant at p 0.05. The results showed that body mass index BMI were higher in females than males 37.20 vs 35.12 kg m2. The oxidative stress markers or parameters of the experimental groups follow a uniform trend of significant increases in Malondialdehyde MDA , and significant decreases in enzymes Glutathione peroxidases GPx and Superoxide dismutase SOD and vitamins C and E . Also middle aged patients 30 50 years were equally affected with diabetes and more in females than males. Generally increased oxidative stress or impaired antioxidant defense system in diabetic obese condition may contribute to increased metabolic damage.

by Egejuru O. Leonard | Akubugwo I. Emmanuel | Chinyere O. Godwin | Ugorji N. Beatrice "Oxidative Stress in Diabetic-Obese Patients Attending Selected Tertiary Hospital in Abia State Nigeria"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021,

URL: https://www.ijtsrd.com/papers/ijtsrd47548.pdf

Paper URL : https://www.ijtsrd.com/biological-science/biochemistry/47548/oxidative-stress-in-diabeticobese-patients-attending-selected-tertiary-hospital-in-abia-state-nigeria/egejuru-o-leonard

openaccessjournalofengineering, engineeringjournal, paperpublicationforengineering

Sunday 19 September 2021

Irregularity of Glycogen Synthase Homeostasis Complications Hepatic and Neuro Defects

Team IJTSRD September 19, 2021 0

Irregularity of Glycogen Synthase Homeostasis Complications Hepatic and Neuro Defects

Glucose homeostasis is strongly regulated to meet the strength needs of major organs and to maintain human health. The liver has a major role in control glucose homeostasis by controlling numerous pathways of glucose metabolism, over eating collection of glycogen union glycogenesis, separate glycogen of glycogenolysis, glucose separate of glycolysis, and under fasting condition amino corrosive into glucose are gluconeogenesis. this metabolic cycle reliant upon admission of food manage eating social huge of our life. Doesnt control influences diabetic, fat, liver sickness and so forth. accumulate facts from hereditary mammal models indicates that the brain, mostly the hypothalamus, has a key major in the homeostatic rule of might and glucose metabolism. Insulin is the activation of glycogen synthase by phosphorylation of the enzyme glucose 6 phosphate ratio. Glycogen is encoded by glycogen synthase, GS1 is expressed in muscle and other tissues, and GS2 is often expressed in the liver, although we have found that GS2 is rarely stored in the brain. We focused liver Glycogen synthase is regulated without delay by glycogen synthase kinase three, AMPK, protein kinase A PKA and casein kinase 2, each protein kinase resulting in a phosphorylated and catalytically inactive glycogen synthase. GS is also regulated by protein phosphatization, which energises glycogen synthase by dephosphorylation. We aimed previous studies on defects of glycogen metabolism.

by Tamilarasan Shanmugasundaram | Uthirakumar Devaraj | Elumalai Balamurugan "Irregularity of Glycogen Synthase Homeostasis Complications- Hepatic and Neuro Defects"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021,

URL: https://www.ijtsrd.com/papers/ijtsrd46479.pdf

Paper URL : https://www.ijtsrd.com/biological-science/biochemistry/46479/irregularity-of-glycogen-synthase-homeostasis-complications-hepatic-and-neuro-defects/tamilarasan-shanmugasundaram

callforpapereconomics, economicsjournal

Thursday 26 August 2021

Comparative Studies of Diabetes in Adult Nigerians Lipid Profile and Antioxidants Vitamins A and C

Team IJTSRD August 26, 2021 0

The study sought to determine the extent to which the usage of social media in the marketing of agricultural products in South West Nigeria can enhance farmers turnover. It employed the survey research design to collect data with the help of a structured questionnaire to elicit information from respondents selected from six 6 south western states. Research data were analysed using structural equation modelling. The results showed that the use of social media WhatsApp and Facebook in marketing of agricultural products significantly enhances farmers turnover. The managerial implication is that use of Whatsapp and Facebook in the marketing of agricultural products for the enhancement of farmers’ turnover was found to have significant influence on the enhancement in farmers’ turnover from agricultural products. Policy makers in government should provide the enabling environment for the telecommunication companies to enhance their reach by installing their facilities across the length and breadth of the country so that the network coverage will be strong at all times so that the benefits of social media usage will not be constrained.

by Egejuru, Leonard O | Akubugwo, Emmanuel I | Ugorji, Beatrice N "Comparative Studies of Diabetes in Adult Nigerians: Lipid Profile and Antioxidants Vitamins (A and C)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021,

URL: https://www.ijtsrd.com/papers/ijtsrd45021.pdf

Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/45021/comparative-studies-of-diabetes-in-adult-nigerians-lipid-profile-and-antioxidants-vitamins-a-and-c/egejuru-leonard-o

callforpaperinternationaljournal, ugclistofjournals, bestinternationaljournal

Tuesday 20 July 2021

Identification of Bioactive Phytochemicals using GC–MS in Leaf Ethanolic Extract of Tragia Involucrata L

Team IJTSRD July 20, 2021 0

Identification of Bioactive Phytochemicals using GC–MS in Leaf Ethanolic Extract of Tragia Involucrata L

Tragia involucrata is belonging to the family Euphorbiaceae common medicinal herb in Southern India. In the present investigation, air died powdered ethanol extracts of Tragia involucrata leaf sample was analyzed by gas chromatography mass spectrometry GC–MS to identify the important phytochemical constituents. The GC MS analysis has shown the presence of 43 active compounds in the leaf extract. Based on area percentage, the top five major compounds present in the ethanolic extract were Palmitic acid 13.39 , 7Z,10Z,13Z 7,10,13 Hexadecatrienal 11.44 , 3beta,24s Stigmast 5 En 3 One 8.10 , Pipeline 7.37 and Friedelan 3 one 6.01 . The GC MS analysis of selected leaf extract proved that the presence of various bioactive compounds. These bioactive compounds justify, the use of this plant to treat various diseases by traditional practitioners.

by Kalaivanan M | A. Saravana Ganthi | M. Padma Sorna Subramanian "Identification of Bioactive Phytochemicals using GC–MS in Leaf Ethanolic Extract of Tragia Involucrata L"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021,

URL: https://www.ijtsrd.compapers/ijtsrd43651.pdf

Paper URL: https://www.ijtsrd.combiological-science/biochemistry/43651/identification-of-bioactive-phytochemicals-using-gc–ms-in-leaf-ethanolic-extract-of-tragia-involucrata-l/kalaivanan-m

callforpaperpharmacy, pharmacyjournal, internationalpeerreviewedjournal

Thursday 30 July 2020

GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Panicum Maximum Jacq Leaves

Team IJTSRD July 30, 2020 0

GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Panicum Maximum Jacq Leaves

hytoconstituents present in the ethanolic extract of Panicum maximum leaves were explored by Gas Chromatography Mass Spectrometry analysis. The compounds were identified by the gas chromatography coupled with the mass spectrometry. The molecular weight and structure of the compounds of Panicum maximum leaves were ascertained by interpretation of the spectrum of GC MS using the database of National Institute of Standard and Technology NIST . GC MS analysis of Panicum maximum leaves revealed the presence of nineteen biological active compounds. The compounds are benzenamine, 4,4 2 4 nitrophenyl ethenylidene bis N,N dimethyl , cyclopropanecarboxylic acid, 1 phenylmethyl , 2,6 bis 1,1 dimethylethyl 4 methylphenyl ester, 4,5 bipyrimidine 2,2 diamine, 4,6 dimethoxy N,N,N,N tetramethyl , 12 Hydroxystearic acid, phenacyl ester, azadirachtin, de E 2 methyl 1 oxo 2 butenyl 1,2 dioxopropyl dihydro , tricyclo 3.3.1.1 3,7 decane, 1,2,2,3,4,4,6,6,8,8,9,9,10,10 tetradecafluoro 5,7 bis trifluoromethyl , spiro cyclohexane 1,3 3H indole 2 carboxy o toluidide, 7 methyl , 1,3,5 triazine 2,4,6 triamine, N,N bis 3 aminophenyl N,N diphenyl ,4 dehydroxy 2,3,3,4,5,6,7 hepta O methylisoorientin, 9,10 anthracenedione, 1,4 bis 2 ethyl 6 methylphenyl amino , l valine, n heptafluorobutyryl , heptadecyl ester, pyrimido 5,4 d pyrimidine, 4,8 di m anisidino 2,6 diethoxy , 2,3,7,7,12,13,17,18 Octaethyl 21H,23H porphine 8 one, 6 fluoro 2 trifluoromethylbenzoic acid, 2 formyl 4,6 dichlorophenyl ester, 2 hydroxyskatole, benzo f cyclopenta a quinolizine 6,7,7a,8,9,10 8H hexacarboxylic acid, 6,7 dihydro , hexamethyl ester, ethyl 2 butyl 3 ethoxycarbonyl methyl amino crotonate , quinoxaline, 2,3 dimethyl , 4H 1 benzopyran 4 one, 5,6 bis acetyloxy 2 3 acetyloxy 4 methoxyphenyl 3,7 dimethoxy It was concluded that the bioactive compounds support the use of Panicum maximum leaves in the treatment of diseases like cancer, diabetes and hypertension.

by O. V. Ikpeazu | I. E. Otuokere | K. K. Igwe "GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Panicum Maximum (Jacq) Leaves"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-5 , August 2020,

URL: https://www.ijtsrd.com/papers/ijtsrd31893.pdf

Paper Url :https://www.ijtsrd.com/chemistry/biochemistry/31893/gc–ms-analysis-of-bioactive-compounds-present-in-ethanol-extract-of-panicum-maximum-jacq-leaves/o-v-ikpeazu

callforpaperbiologicalscience, biologicalsciencejournal

Friday 22 May 2020

Endothelial Nitric Oxide Synthase T786C Gene Polymorphism TT Genotype is Associated with High Nitric Oxide Levels and Low HOMA IR Levels in Coronary Artery Disease Patients

Team IJTSRD May 22, 2020 0

Endothelial Nitric Oxide Synthase T786C Gene Polymorphism TT Genotype is Associated with High Nitric Oxide Levels and Low HOMA IR Levels in Coronary Artery Disease Patients

BACKGROUND Data suggest eNOS 786T C gene polymorphism is distinct in specific population group, ethnicity and geographic region and perhaps this genetic variability might produce different results on exposure to various environmental factors 9 14. Besides there is hardly any data available in Indian population. Sofurther research is needed to explore the complex interaction between environmental factors and eNOS 786T C gene polymorphism in susceptibility to insulin resistance in patients with CAD in Indian population.AIM This study aimed to study the association of Insulin Resistance, Nitric Oxide and intergenotypic variation of endothelial Nitric Oxide Synthase T786C gene polymorphism among coronary artery disease subjects. METHODOLOGY This study consisted of 60 adult patients of with documented CAD and 60 age and sex matched healthy subjects as controls.Fasting Serum Insulin was measured by ELISA,fasting Serum nitric oxide by modified Griess reaction and fasting plasma glucose on fully automated chemistry analyzer Hitachi 902 HOMA IR was calculated mathematically by using formula given by Matthew et al. The eNOS gene loci was amplified by using PCR and by RFLP. A p value 0.05 was considered significant. Statistical analysis was performed with the help of SPSS version.. RESULTS Intergenotypic levels of nitric oxide were significantly lower in TC genotype than TT both in study as well as control groups. Also, TC genotype showed significantly higher HOMA IR levels as compared to TT genotype in both study as well as control group. Moreover, CC genotype could not be found in both cases and control groups..Thus, TT genotype may have a protective role in development of insulin resistance and coronary artery disease as seen by high Nitric Oxide levels and low HOMA IR levels in TT genotype than TC genotypes in both case as well as control groups.CONCLUSIONS Endothelial nitric oxide synthase t786c gene polymorphism tt genotype is associated with high nitric oxide levels and low homa ir levels in coronary artery disease patients.

BY Namrata Bhutani | Deepak Tangadi | Neha Bhutani "Endothelial Nitric Oxide Synthase (T786C) Gene Polymorphism TT Genotype is Associated with High Nitric Oxide Levels and Low HOMA-IR Levels in Coronary Artery Disease Patients"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020,

URL: https://www.ijtsrd.com/papers/ijtsrd31317.pdf

Paper Url :https://www.ijtsrd.com/biological-science/biochemistry/31317/endothelial-nitric-oxide-synthase-t786c-gene-polymorphism-tt-genotype-is-associated-with-high-nitric-oxide-levels-and-low-homair-levels-in-coronary-artery-disease-patients/namrata-bhutani

openaccessjournalofengineering, engineeringjournal, paperpublicationforengineering

Thursday 21 May 2020

Role of Pre Therapeutic Expression of Human Organic Cation Transporter1 SLC22A1 Gene in Predicting Clinical Outcome in Imatinib Treated CP CML Patients

Team IJTSRD May 21, 2020 0

Role of Pre Therapeutic Expression of Human Organic Cation Transporter1 SLC22A1 Gene in Predicting Clinical Outcome in Imatinib Treated CP CML Patients

Background Human organic cation transporter1 hOCT1, SLC22A1 , an influx transporter,is responsible for the uptake of Imatinib into chronic myelod leukemia CML cells. Some patients fail to achieve optimal molecular response to Imatinib, defined as major molecular response MMR i.e.BCR ABL 1 = 0.1 within 12 months of therapy. Pretherapeutic expression of hOCT1 may be beneficial in predicting the response to imatinib in CML patients. Methodology 30 newly diagnosed BCR ABL positive CML patients in chronic phaseand30 healthy control subjects, all ethnic Indians ,were recruited in the study. hOCT1 gene expression in PBMCs was quantified by SYBR Green based qRT PCR, using the 2 DDCt method. After initiation of imatinib therapy, hematological response was monitored at regular intervals, and molecular response BCR ABL1 ABL1 ratio assessed after 6 or 12 months. Results and discussion The cases were divided into two groups, high expression n=15 and low expression n=15 groups, based on the median value of fold change in hOCT1 gene expression. Median =5.6 . 11 73.33 patients with low expression achieved CHR by the end of 3 months, whereas 4 26.66 did not. On the contrary, all 15 patients 100 with high hOCT1 gene expression achieved CHR by the end of 3 months. p=0.10 .It was also observed that the mean THR time to CHR in low expression group was higher than in high expression group. p=0.046 It was seen that while all 15 patients with high expression had an optimal response , only 13.33 n=2 patients with low expression had it. In the low expression group, 40 patients had treatment failure n=6 while remaining 23.33 n=7 were categorized as warning. p=0.000 ELN 2013 guidelines Conclusions Hence it was concluded that high expression of hOCT1 gene leads to early achievement of CHR. In case of molecular response, it was observed that high expression of hOCT1 gene was significantly associated with achievement of on optimal response to imatinib. These findings emphasize that knowledge of pretherapeutic level of hOCT1 could be a useful marker to predict imatinib therapy outcome in CML patients, and the prospects of personalized therapy in such patients.

BY Namrata Bhutani | Deepika Arora | Neha Bhutani "Role of Pre-Therapeutic Expression of Human Organic Cation Transporter1 (SLC22A1) Gene in Predicting Clinical Outcome in Imatinib Treated CP-CML Patients"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020,

URL: https://www.ijtsrd.com/papers/ijtsrd31316.pdf

Paper Url :https://www.ijtsrd.com/biological-science/biochemistry/31316/role-of-pretherapeutic-expression-of-human-organic-cation-transporter1-slc22a1-gene-in-predicting-clinical-outcome-in-imatinib-treated-cpcml-patients/namrata-bhutani

internationaljournalsinengineering, callforpaperengineering, ugcapprovedengineeringjournal

Tuesday 10 March 2020

Heavy Metals Level Evaluation in Lycopersicon Esculentum Tomato and Daucus Carota Carrot Cultivated In Two Farming Communities in Kazaure Local Government Area

Team IJTSRD March 10, 2020 0

Heavy Metals Level Evaluation in Lycopersicon Esculentum Tomato and Daucus Carota Carrot Cultivated In Two Farming Communities in Kazaure Local Government Area

The exposure to heavy metals such as Ni, Cd, Cr, Co, Pb, As, Hg, Zn, Fe and Cu, has been reported as a risk to human health through the consumption of vegetable when exposed to air pollution and other environmental and agricultural pesticides. This paper is aimed to evaluate the level of heavy metal in carrot and tomatoes cultivated in two farming communities in Kazaure Local Government Area, Jigawa State, Nigeria. The heavy metals were analysed using Atomic Absorption Spectroscopy. The result showed that the mean levels concentration of heavy metals from carrot and tomatoes fruit cultivated from the two farming communities in Kazaure namely Gada and Firji farming areas. The heavy Ni, Cd, Fe, Pb, Mn, Zn and Cr concentration for carrots cultivated at Gada farming area were ranged from 0.06 0.09 mg kg, 0.08 0.09 mg kg, 8.24 8.91 mg kg,0.13 0.23 mg kg,1.23 ,1.56 1.67 mg kg and 0.02 0.04 mg kg respectively. The heavy metals As Cu and Co were not detected in the fruits of carrot for cultivated in both two farming areas. While the mean concentration obtained from tomatoes fruits from the Gada and Firji farming areas ranged from 0.14 0.16 mg kg, 0.06 0.07 mg kg, 6.11 6.12 mg kg, 0.97 0,99 mg kg, 1.11 1.23 mg kg, 0.35 0.39 mg kg for Ni, Cd, Fe, Mn, Zn and Cr respectively. While As, Cu, Pb and Co were not found in tomatoes harvested from the two farming communities. The study concludes that the level of some heavy metals analysed in carrot and tomatoes fruits were found within the permissible level except lead and cadmium.

by Ibrahim M. D | Abdulmumin Y | Abdulmumin T. M | Adamu A. U ""Heavy Metals Level Evaluation in Lycopersicon Esculentum (Tomato) and Daucus Carota (Carrot) Cultivated In Two Farming Communities in Kazaure Local Government Area""

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-3 , April 2020,

URL: https://www.ijtsrd.com/papers/ijtsrd30269.pdf

Paper Url :https://www.ijtsrd.com/biological-science/biochemistry/30269/heavy-metals-level-evaluation-in-lycopersicon-esculentum-tomato-and-daucus-carota-carrot-cultivated-in-two-farming-communities-in-kazaure-local-government-area/ibrahim-m-d

callforpaperpapersinjournals, multidisciplinaryjournal

Wednesday 27 November 2019

GC/MS Analysis and Evaluation of Antimicrobial Performance of Aframomum Latifolium Leaf Essential Oil from South West Nigeria

Team IJTSRD November 27, 2019 0

GC/MS Analysis and Evaluation of Antimicrobial Performance of Aframomum Latifolium Leaf Essential Oil from South West Nigeria

This study was conducted to analyze the chemical constituent and to evaluate the antimicrobial properties of the leaf essential oil of Aframomum latifolium. The leaves of this plant was subjected for hydro distillation using all glass Clevenger apparatus. The volatile oil obtained was analysed by means of gas chromatography mass spectrometry GC Ms . The oil yield was 0.08 v w. Seventeen components were identified in the leaf essential oil accounting for 99.90 of the total oil fraction. The leaf oil was dominated by oxygenated monoterpenes 51.26 and monoterpenes 36.22 . Cajeputol, an oxygenated monoterpene is the component with the highest percentage composition 45.05 , cyclofenchene 7.82 . Peperitone is the component with the lowest percentage 0.43 . The oil displayed high antimicrobial Potentials to Staphylococos aureus, Escherichia Coli, Bacillus subtilis, Pseudomonas aeruginosa, Candida albican and aspergillus niger at 100 , 50 and 25 and a moderate inhibition at 12.5 and 6.25 The result obtained from this study justifies the use of the leaf of this plant in ethnomedical practices.

by Ogunmola Oluranti Olagoke | Amusat Mumini A. ""GC/MS Analysis and Evaluation of Antimicrobial Performance of Aframomum Latifolium Leaf Essential Oil from South West Nigeria""

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019,

URL: https://www.ijtsrd.com/papers/ijtsrd29481.pdf

Paper URL: https://www.ijtsrd.com/chemistry/biochemistry/29481/gcms-analysis-and-evaluation-of-antimicrobial-performance-of-aframomum-latifolium-leaf-essential-oil-from-south-west-nigeria/ogunmola-oluranti-olagoke

call for paper biological science, ugc approved journals for computer science, biological science journal

Tuesday 1 October 2019

Comparative Study of Free Radical Scavenging and Lipid Peroxidation Inhibition Potentials of Oxythenanthera Abyssinica Rhizomes Sourced from South Eastern Nigeria

Team IJTSRD October 01, 2019 0

Comparative Study of Free Radical Scavenging and Lipid Peroxidation Inhibition Potentials of Oxythenanthera Abyssinica Rhizomes Sourced from South Eastern Nigeria

Oxytenanthera abyssinica rhizomes have been used in the treatment of aliments varying from colics, diabetes, polyuria and albuminiura, these ethanomedicinal potencies have been attributed to its rich phytochemical contents and free radical scavenging capacity. This work is aimed at investigating the effect of crude oil pollution on total phenolic, flavonoids and free radical scavenging potentials of methanol extract of Oxytenanthera abyssinica rhizomes. The ability of the plant extracts to scavenge 2,2 diphenyl 1 picylhydrazyl DPPH , hydroxyl radical .OH , superoxide radical O2 , nitric oxide radical NO. and ability to inhibit lipid peroxidation was assayed following standard methods, spectrophotometric method was used to assay for total phenolic and flavonoid content. Non crude oil polluted O. abyssinica extract NCOPOAE and crude oil polluted O. abyssinica extract COPOAE in a concentration dependent manner showed a potent antioxidant activity in DPPH radical scavenging assay EC50= 1.03 ±0.01µg ml and EC50 = 1.04 ±1.01µg ml , inhibited hydroxyl radical EC50 = 1.17 ±1.02µg ml and EC50 = 1.46 ±1.21µg ml , superoxide anion radical EC50 =1.30 ±1.21µg ml and EC50 =1.51 ±1.42µg ml and nitric oxide EC50 = 1.40 ±1.23µg ml and EC50= 1.53 ±1.42µg ml also inhibited non enzymatic lipid peroxidation EC50 = 1.58 ±1.42µg ml and EC50 = 2.47 ±2.13µg ml and had more of phenolic and flavonoid contents than COPOAE. This result indicates that NCOPOAE contains more antioxidant compounds than COPOAE, which could make it more potent in ethanomedicinal use.

by Jonathan Chinenye Ifemeje | Kizito Ifeanyi Amaefule | Charles Tochukwu Nwachukwu "Comparative Study of Free Radical Scavenging and Lipid Peroxidation Inhibition Potentials of Oxythenanthera Abyssinica (Rhizomes) Sourced from South Eastern Nigeria"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-6 , October 2019,

URL: https://www.ijtsrd.com/papers/ijtsrd29226.pdf

Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/29226/comparative-study-of-free-radical-scavenging-and-lipid-peroxidation-inhibition-potentials-of-oxythenanthera-abyssinica-rhizomes-sourced-from-south-eastern-nigeria/jonathan-chinenye-ifemeje

arts journal, paper publication in management, call for paper life sciences

Tuesday 16 July 2019

Effect of crude oil pollution on phytochemical and anti diabetic potentials of Oxytenanthera abyssinica rhizomes in normal and alloxan induced diabetic rats

Team IJTSRD July 16, 2019 0

Effect of crude oil pollution on phytochemical and anti diabetic potentials of Oxytenanthera abyssinica rhizomes in normal and alloxan induced diabetic rats

Objectives This study is aimed at investigating the effect of crude oil pollution on the phytochemical content and anti diabetic potentials of Oxytenanthera abyssinica rhizomes in normal and alloxan induced diabetic rats using standard methods. Method The ability of the extracts to reduce blood glucose level in normal rats was done using 200 and 400 mg kg b.w of non crude oil polluted Oxytenanthera abyssinica extract NCOPOAE and 200 and 400 mg kg b.w of crude oil polluted Oxytenanthera abyssinica extract COPOAE respectively. Alloxan monohydrate at 130 mg kg b.w was used to induce diabetes to rats and was treated with 100, 200 and 400 mg kg b.w of NCOPOAE and 100, 200 and 400 mg kg b.w of COPOAE respectively while phytochemical analysis was done following standard method. Results Oral glucose tolerance test revealed significant decrease in plasma glucose concentration p 0.05 in groups of rats fed 200 and 400 mg kg b.w of NCOPOAE at intervals of 30, 60 and 120 minutes while that of COPOAE, none significantly decreased the plasma glucose concentration, when compared with those of control groups. The extracts did not have any significant effect in alloxan induced diabetic model. Conclusion These results could indicate that crude oil pollution could affect the synthesis of phytochemicals in plants therefore affecting the ethnomedicinal potencies. It also shows that O. abyssinca rhizomes could be more potent in management of type 2 diabetes where the cells are resistant to insulin.

by Kizito Ifeanyi Amaefule | Jonathan Chinenye Ifemeje | Chinenye Azuka Mere ""Effect of crude oil pollution on phytochemical and anti-diabetic potentials of Oxytenanthera abyssinica (rhizomes) in normal and alloxan induced diabetic rats""

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019,

URL: https://www.ijtsrd.com/papers/ijtsrd26557.pdf

Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/26557/effect-of-crude-oil-pollution-on-phytochemical-and-anti-diabetic-potentials-of-oxytenanthera-abyssinica-rhizomes-in-normal-and-alloxan-induced-diabetic-rats/kizito-ifeanyi-amaefule

ugc approved journals for management, mathematics journal

Tuesday 16 April 2019

Critical Analysis of Wheat Bran as Therapeutic Source

Team IJTSRD April 16, 2019 0

Critical Analysis of Wheat Bran as Therapeutic Source

Increasing population now a days is one of the most crucial phases of upcoming future troubles. Food wastage along with the nutritional expenditure should not be entertained. Several milling by products of crops viz., wheat bran, are principle pool of nutrients. It is very much essential to study and gain knowledge about the nutrients, anti oxidants, anti nutritional components and their effect upon diseased condition along with the probability of production of edible food stuffs for human. Though there is enough production of wheat bran, most of the amount is either wasted or used as livestock fed. It has been shown that bran part has potential efficiency to be used as therapeutic tool for various diseases such as, cancer, obesity and diabetes, bowel irritation etc. Its role as a prebiotic might also prove an asset to remarkably influence gut microflora. Its anti nutritional content is also lower than the rice bran and that is also manageable for the further use as food stuff. The physicochemical properties along with its nutrient as well as anti nutrient contents must be utilized and regulated as requirement to combat malnourishment besides hunger issues and therapies.

by Manali Chakraborty | Dr Savita Budhwar "Critical Analysis of Wheat Bran as Therapeutic Source"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3, April 2019,

URL: https://www.ijtsrd.com/papers/ijtsrd21755.pdf

Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/21755/critical-analysis-of-wheat-bran-as-therapeutic-source/manali-chakraborty

call for paper chemistry, ugc approved journals for chemistry, chemistry journal

Thursday 4 April 2019

Study of Glutathione Peroxidase GPX Activity Among Betel Quid Chewers of Indian Population

Team IJTSRD April 04, 2019 0

Study of Glutathione Peroxidase GPX Activity Among Betel Quid Chewers of Indian Population

Introduction Betel quid BQ chewing, a habit practiced in Eastern and North Eastern part of India, has known to be associated with cancer of the oral or buccal cavity. BQ is also one of the common mood elevating substances among Indian population. The BQ is a mixture of areca nut Areca catechu, catechu Acacia catechu and slaked lime calcium oxide and calcium hydroxide wrapped in a betel leaf Piper betel .BQ products have been classified by the International Agency for Research on Cancer IARC as group I human carcinogens. Glutathione peroxidise GPx, one of the major enzymatic antioxidant defence system, responsible for scavenging free radicals. Antioxidant enzymes catalyze decomposition of ROS.

Overall balance between production and removal of ROS may be more important in various cancers including OSCC Oral squamous cell carcinoma or oral cancer. Methods In this study subjects were screened from Department of Oral and Maxillofacial surgery andE.N.T. of Ramakrishna Mission Seva Pratishthan Hospital RKMSP, Kolkata and different areas of West Bengal and North Eastern states of India. Quantitative in vitro determination of glutathione peroxidase activities in whole blood were estimated manually with 0.05 ml whole blood. The samples were assayed by UV Visible Spectrophotometer SPECORD 50 PLUS at a wavelength of 340nm. Results Most of the subjects had betel quid chewing habit. Glutathione peroxidase values are higher in healthy control than Cancer cases and Pre cancer with betel quid chewing habit, which is statistically significant. Conclusion Reactive oxygen species are generated due to slaked lime, one of the important constituents of betel quid which can modulate the oral pathology and promote carcinogenesis.

by Aniket Adhikari | Madhusnata De "Study of Glutathione Peroxidase (GPX) Activity Among Betel Quid Chewers of Indian Population"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3, April 2019,

URL: https://www.ijtsrd.com/papers/ijtsrd21619.pdf

Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/21619/study-of-glutathione-peroxidase-gpx-activity-among-betel-quid-chewers-of-indian-population/aniket-adhikari

call for paper chemistry, ugc approved journals for chemistry, chemistry journal

Wednesday 10 October 2018

Effect of Methanol Leaf Extract of Vitex Doniana on Cadmium Chloride-Induced Toxicity in Kidney and Liver Tissues of Male Wistar Rats

IJTSRD October 10, 2018 0

Effect of Methanol Leaf Extract of Vitex Doniana on Cadmium Chloride-Induced Toxicity in Kidney and Liver Tissues of Male Wistar Rats

The aim of this study was to investigate the effects of methanol leaf extract of Vitex doniana MEVd on Cadmium chloride - induced toxicity in the liver and kidney of male Wistar rats was investigated. Twenty five male Wistar rats weighing 171.87 Â± 7.86g were acclimatized for two weeks and subsequently randomized into five groups Group 1 rats were administered daily 1 ml distilled water control group group 2 were administered Cadmium chloride 10 mg kg b.w. only groups 3 and 4 were administered MEVd 200 and 400 mg kg b.w. respectively six hours prior administration of Cd 10 mg Cd kg b.w. and group 5 rats were administered MEVd 400 mg kg b.w. only. The experiment lasted six weeks and all administration was carried out orally.

The final body weights of the rats were recorded at the end of the experiment and afterwards the rats were sacrificed, blood was collected and the serum was subsequently prepared. The kidneys and liver were excised, weighed and then stored in 10 formalin for histological examination. The serum was used for the determination of urea, creatinine, Na , K , and Cl- concentrations, aspartate aminotransferase AST and alanine aminotransferase ALT activities and total bilirubin T-Bil concentration. The results showed that exposure of the rats to Cd significantly p 0.05 increased kidney weight, serum urea, creatinine, Na , K , Cl- and total bilirubin concentrations as well as serum ALT and AST activities.

Cd-induced toxicity also caused severe necrosis of the tubular epithelial cells in the nephrons and degeneration of the hepatocytes. Although both doses of the plant extract significantly ameliorated these changes, the amelioration at 200 mg kg body weight of the extract compared well with the control in most of the parameters investigated. In conclusion, the oral administration of methanol leaf extract of Vitex doniana ameliorated the Cd-induced toxicity related biochemical parameters and histological changes in the rats.

by Olajide, J. E. | Sanni, M. | Omattah, G. O. "Effect of Methanol Leaf Extract of Vitex Doniana on Cadmium Chloride-Induced Toxicity in Kidney and Liver Tissues of Male Wistar Rats"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-6 , October 2018,

URL: http://www.ijtsrd.com/papers/ijtsrd18876.pdf

Direct Link: http://www.ijtsrd.com/biological-science/biochemistry/18876/effect-of-methanol-leaf-extract-of-vitex-doniana-on-cadmium-chloride-induced-toxicity-in-kidney-and-liver-tissues-of-male-wistar-rats/olajide-j-e

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Wednesday 1 August 2018

Prunin Production From Orange Peel Naringin Hydrolyzed By a-L-rhamnosidase from Aspergillus Flavipus MTCC- 4644

IJTSRD August 01, 2018 0

Prunin Production From Orange Peel Naringin Hydrolyzed By a-L-rhamnosidase from Aspergillus Flavipus MTCC- 4644

An orange (Citrus sinensis) is the most common fruits in the world. The wastes generated from the orange fruit needs to be put in to beneficial use. In this study the primary wastes (peel) of orange is use for preparation of prunin. a-L-Rhamnosidase (EC 3.2.1.40) secreted by Aspergillus flavipus MTCC-4644 are potential catalysis in hydrolysis of naringin content present in orange peels. a-L-rhamnosidase from the culture filtrate of a fungal strain, Aspergillus flavipus MTCC-4446 has been purified to homogeneity. The procedure involved concentration by ultra filtration and cation-exchange chromatography on car boxy methyl cellulose.

The purified enzyme gave a single protein band corresponding to molecular mass of 40.0 kDa in SDS-PAGE analysis showing that the enzyme preparation was pure. The native PAGE analysis of the purified enzyme also gave single protein band confirming the purity of the enzyme preparation. Using p-nitro phenyl -a-L-rhamnopyranoside as substrate, Km and kcat values of the enzyme were 0.48 mM and 28. 4 s-1 respectively. The pH and temperature optima of the enzyme were 5.0 and 50 Â°C, respectively. The enzyme is stable below10ÂºC and at pH 4.5. The energy of activation for thermal denaturation of enzyme determined by Arrhenius plot was 32.06 k J mol-1.The enzyme hydrolyzed naringin content of orange peel to L-rhamnose and prunin.

By Sarita Yadav" Prunin Production From Orange Peel Naringin Hydrolyzed By a-L-rhamnosidase from Aspergillus Flavipus MTCC- 4644"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018,

Paper URL: http://www.ijtsrd.com/papers/ijtsrd16969.pdf

Direct URL: http://www.ijtsrd.com/chemistry/biochemistry/16969/prunin-production-from-orange-peel-naringin-hydrolyzed-by-a-l-rhamnosidase-from-aspergillus-flavipus--mtcc--4644/sarita-yadav

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Tuesday 1 May 2018

Biochemical Parameters of Blood Cows at Hight and Low Temperatures

IJTSRD May 01, 2018 0

Biochemical Parameters of Blood Cows at Hight and Low Temperatures

Biochemical analysis of blood is a complex laboratory diagnosis conducted to assess the condition of internal organs and systems and to identify the body's need for microelements and the level of its satisfaction. According to the biochemical parameters of the blood composition, primary diagnostics of the functioning of the liver, kidneys, pancreas and other organs is carried out, and data on metabolic processes (lipid, albuminous, carbohydrate metabolisms) are obtained.

High and low temperature have a great influence on the physiological processes in the animals, on their thermal state, health, productive qualities, the use of feed, which ultimately determines the efficiency of the work of livestock farms. Season profoundly affected all biochemistry parameters, possibly due to changes in nutrition and disease exposure. Air temperature affects the heat exchange functions of cows. Adverse effects on the body have both a low and high temperature, and especially its sharp fluctuations.

Low temperature enhances heat production due to large feed intake by animals, as well as adaptation processes in animals.Biochemical blood tests are very important for the timely diagnosis of various pathological conditions. Currently, veterinary laboratories in the sample of one animal determine several indicators at once, so the introduction of automatic analyzers is of great importance.

by Ward M. Ashraf | Abuargob M. Omry | Hdud M. Ismail | Elgusbi M. Tarek | Ruban Y. Sergey "Biochemical Parameters of Blood Cows at Hight and Low Temperatures"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018,

URL: http://www.ijtsrd.com/papers/ijtsrd12960.pdf

Direct Link - http://www.ijtsrd.com/chemistry/biochemistry/12960/biochemical-parameters-of-blood-cows-at-hight-and-low-temperatures/ward-m-ashraf

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Thursday 4 January 2018

Clinical Applications of Metabolomics in Diabetes Research

IJTSRD January 04, 2018 0

Clinical Applications of Metabolomics in Diabetes Research

Diabetes mellitus is a multi-factorial disorder related to a dysregulated metabolism. a holistic technique emphasizing each of the person factors with regards to finish pathophysiology of the disease is vital to our information of this heterogeneous disorder.

numerous technical advances inside the area of purposeful genomics which include metabolomics and proteomics useful resource in comprehending the nation of the overall organic machine and for this reason can be applied to decipher the complicated interactions among additives of the metabolic machine in human diabetes. above-referred to strategies combined with a fixed of bioinformatics equipment and available databases goal to profile big selection of proteins and metabolites repertoire in humans.

the accurate and comprehensive measurements of those molecules is hired to analyze complicated interactions of metabolites and proteins now not simplest amongst themselves but additionally with genes, transcripts and other small molecules to decipher cell microenvironment and the results due to drug intervention. this assessment gives an overview of the applications of metabolomics in human diabetes studies.

we additionally talk the ability of combining exceptional bioinformatics tools with the omics technique to improve the medical expertise closer to discovery of biomarkers to monitor and adjust general human fitness with appreciate to the deregulated metabolic state, a function of diabetes mellitus.

by Seema Ahad "Clinical Applications of Metabolomics in Diabetes Research"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018,

URL: http://www.ijtsrd.com/papers/ijtsrd8360.pdf

Direct Link - http://www.ijtsrd.com/biological-science/biochemistry/8360/clinical-applications-of-metabolomics-in-diabetes-research/seema-ahad

Biochemistry, Engineering Journal

Friday 3 November 2017

A study on antioxidants (vitamin c & e) in cannabis user in diabetic and non-diabetic subjects

IJTSRD November 03, 2017 0

A study on antioxidants (vitamin c & e) in cannabis user in diabetic and non-diabetic subjects

Aim & Objectives: The correlation of Cannabis user groups with duration of one-month, one-year and five year (Both diabetic and non-diabetic groups) with serum Vitamin C level and serum vitamin E level.Research Design and Methods: In a cross-sectional, case-control study, we studied cannabis user 55 and 50 cannabis non-user, male-subjects aged between 25 and 65 years. Fasting blood samples were obtained from 55 diabetic male subjects and 50 healthy control subjects.

Blood samples were withdrawn who were taking 5 to 10 gm of cannabis per day and analyzed for Antioxidant serum Vitamin C and serum vitamin E by standard technique using UV-Spectrophotometer. Results: serum Vitamin C and serum vitamin E were measured and the results were compared with those of controls.

Antioxidants levels were found to be significantly higher in diabetic and non-diabetic subjects using cannabis 5 to 10 gm per day for the last one month, one year and five years when compare with normal control and diabetic control subjects(p <0.01).Conclusion: Study shows a significant increased in Vitamin C and serum vitamin E level recorded diabetic patients and non diabetic groups using cannabis for the last one year and five year as well as in the whole group as compared to that of normal control.

It might be possible that either cannabis may enhanced the insulin release or increase the penetration of sugar into the cells and might be due to antioxidant activity of cannabis.

By Dr. Ghanshyam Gahlot | Dr. Yogita Soni "A study on antioxidants (vitamin c & e) in cannabis user in diabetic and non-diabetic subjects"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1 , December 2017,

URL: http://www.ijtsrd.com/papers/ijtsrd5875.pdf

Direct URL: http://www.ijtsrd.com/biological-science/biochemistry/5875/a-study-on-antioxidants-vitamin-c-and-e-in-cannabis-user-in-diabetic-and-non-diabetic-subjects/dr-ghanshyam-gahlot

Indexed Journal, Biochemistry Journal

Sunday 21 February 2016

Pharmacological Applications of Isorhamnetin: A Short Review

IJTSRD February 21, 2016 0

Pharmacological Applications of Isorhamnetin: A Short Review

Isorhamnetin (30-Methoxy-3, 40, 5, 7-tetrahydroxyflavone) is a flavonol, and it belongs to a group of plant secondary metabolites known as flavonoids. It is present in man's daily diet and is known for its biological activities such as antioxidant, antiviral, anticancer, antimicrobial, and anti-inflammatory effects. Isorhamnetin has been reported for its antioxidant and antiviral applications hence it is not only used as such, but it also has various derivatized forms which has potentials for development into drugs for the treatment of diseases caused by oxidative stress and lethal viruses. The present review reflects the pharmacological importance of isorhamnetin.

By S Kandakumar | Dr V Manju" Pharmacological Applications of Isorhamnetin: A Short Review"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-4 , June 2017,

Paper URL: http://www.ijtsrd.com/papers/ijtsrd2202.pdf

Direct URL: http://www.ijtsrd.com/biological-science/biochemistry/2202/pharmacological-applications-of-isorhamnetin-a-short-review/s-kandakumar

UGC Approved Journal Biochemistry Journal

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