International Journal of Trend in Scientific Research and Development: Cell biology

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Showing posts with label Cell biology. Show all posts
Showing posts with label Cell biology. Show all posts

Saturday 15 February 2020

A Reliable Technique for Stem Cell Marker Identification

February 15, 2020 0
A Reliable Technique for Stem Cell Marker Identification
Stem cells have emerged to give a promising result in regenerative medicine. Stem cell derived growth have been studied in various animals. These stem cell are identified by specific markers. Various methods have been used to identify these markers. Some of the methods were been used last decades to identify the stem cells. This article presents a concise review on the methods used for identifying markers and discuss the very most reliable technique of the identification of stem cell markers. 

by Dr. Sai Charan. K. V | Dr. Nirmal Famila Betty ""A Reliable Technique for Stem Cell Marker Identification""

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-2 , February 2020,

URL: https://www.ijtsrd.com/papers/ijtsrd30146.pdf

Paper Url : https://www.ijtsrd.com/biological-science/cell-biology/30146/a-reliable-technique-for-stem-cell-marker-identification/dr-sai-charan-k-v

ugcapprovedjournalswithlowpublicationfees, conferenceissuepublication

Thursday 2 January 2020

Analyze the Molecular Genetics of a Solitary Causative Genetic Material behind Muscular Dystrophy

January 02, 2020 0
Analyze the Molecular Genetics of a Solitary Causative Genetic Material behind Muscular Dystrophy

Muscular dystrophy is a genetic disorder characterized by progressive muscle weakness and degeneration. Duchenne muscular dystrophy DMD is the most common X linked disorder muscular dystrophy. It is caused by an absence of the protein named as dystrophin, which helps to keep muscle cells intact. In my research on this topic, I will emphasis on a complete analysis of molecular gene behind muscular dystrophy. According to a survey with few hospitals labs in my region with 40 50 patients, we had identified the ratio of around 90 of male and 10 of the female victim with the muscular dystrophy disorder. The lifespan of people having this disorder decreases unconditionally and it majorly effect the youngsters. Main concern for the treatment methodology or medical interventions are limited to treating symptoms. 


by Nishtaa Modi ""Analyze the Molecular Genetics of a Solitary Causative Genetic Material behind Muscular Dystrophy""

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-2 , February 2020,

URL: https://www.ijtsrd.com/papers/ijtsrd29850.pdf

Paper Url : https://www.ijtsrd.com/biological-science/cell-biology/29850/analyze-the-molecular-genetics-of-a-solitary-causative-genetic-material-behind-muscular-dystrophy/nishtaa-modi

callforpaperpapersinjournals, multidisciplinaryjournal

Friday 12 April 2019

Cultivation of Two Species of Mushroom Found in Anambra State, South Eastern, Nigeria and Their Potentials for Bioremediation of Trace Metals Polluted Soil

April 12, 2019 0
Cultivation of Two Species of Mushroom Found in Anambra State, South Eastern, Nigeria and Their Potentials for Bioremediation of Trace Metals Polluted Soil

Two species of Mushroom namely Amanita phalaoides and Amanita verosa were studied and they were collected from farmlands in Uke town around Onitsha metropolis in Anambra State, Nigeria. The mushroom samples were cultivated on normal agricultural and refuse dump soils. The young growing mushrooms were watered twice daily and harvested within fourteen 14 days. The harvested mushroom samples were sun dried and kept in pre cleaned bottles for chemical analysis. The dried samples were cut into pieces and pulverized using binatone blender with stainless blade and they were subjected to chemical analysis using standard analytical methods AOAC . The obtained data were analyzed by ANOVA using SPSS versions 16.0. Duncans Range was applied in separating means where differences were observed. The results ranged as follows moisture content MC 81.79 to 97.84 , the highest value was from Amanita Phalaoides. Dry matter DM ranged from 2.63 to 18.36 shows an indication of high roughages contained by mushrooms. Crude protein CP ranged from 8.16 to 24.67 which compared favourably with values of seeds and legumes. Ash contents ranged from 3.26 to 14.33 and they are indications of high mineral elements present in mushroom species. Low values of Lipid fat oil ranged from 1.00 to 6.68 are indications that mushrooms are excellent dietary food for diabetic and coronary heart disease patients. Crude fibre CF ranged from 2.62 to 15.37 . Values of Ethanol soluble sugar ESS , carbohydrate CHO and vitamin C were close showing no significant difference at p 0.05. Values of phytic acid, tannins and hydrocyanic acid ranged from 0.01 to 0.27 mg 100g, these were quite low to give adverse effect. The mean concentrations of nutrients metals mg kg of Na, K, Ca, Mg and Fe ranged from 276.48±0.27 to 826.83±0.04 while trace metals Cu, Co, Pb, Zn, Cd, Ni, Mn, Cr concentrations mg kg ranged from 0.001±0.01 to 43.18±0.20. Cobalt was most bio accumulated in agric soil while Zn was most bio accumulated in refuse dump soil. 


by Dr. (Mrs) B. C. Ilechukwu | Prof. C. O. B. Okoye "Cultivation of Two Species of Mushroom Found in Anambra State, South-Eastern, Nigeria and Their Potentials for Bioremediation of Trace Metals Polluted Soil"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019,

URL: https://www.ijtsrd.com/papers/ijtsrd22846.pdf

Paper URL: https://www.ijtsrd.com/biological-science/cell-biology/22846/cultivation-of-two-species-of-mushroom-found-in-anambra-state-south-eastern-nigeria-and-their-potentials-for-bioremediation-of-trace-metals-polluted-soil/dr-mrs-b-c-ilechukwu

call for paper pharmacy, pharmacy journal, international peer reviewed journal


Wednesday 30 January 2019

Forensic Existence of Blood as a Dynamic Evidence

January 30, 2019 0
Forensic Existence of Blood as a Dynamic Evidence

Blood is one of the chief form of biological fluid which is recovered at the location of crime. This review article focus on blood as a prime forensic evidence which is most commonly encountered on crime scenes. The font of insipiration for the same is the variable facts and the frequent occurrence of blood as an evidence on the crime scene. The article pronounces the complete details of blood, including its composition, cell types, relation of blood to scientific science, handling liquid blood stains , wet blood stains, dried blood stains, and the post mortum blood and preservation methods followed by a detailed analysis preliminary and confirmatory test . The analysis of blood can be categorised on the basis of its physical and chemical properties. The major aim of this study is to provide all the relevant facts related to blood collection, packaging and the entire examination. 


BY Sanya Sharma | Shipra Rohatgi "Forensic Existence of Blood as a Dynamic Evidence"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , Febru
ary 2019,

URL: https://www.ijtsrd.com/papers/ijtsrd21396.pdf

Paper URL: https://www.ijtsrd.com/biological-science/cell-biology/21396/forensic-existence-of-blood-as-a-dynamic-evidence/sanya-sharma

call for paper social science, ugc approved journals for social science, social science journal

Monday 11 December 2017

Lipopolysaccharide promotes the metastatic Potential of Lung Carcinoma Cells by Upregulating the Expression of Fibronectin

December 11, 2017 0
Lipopolysaccharide promotes the metastatic Potential of Lung Carcinoma Cells by Upregulating the Expression of Fibronectin
Lipopolysaccharide (LPS) stimulated TLR-4 mediated signalling has been shown to accentuate the metastasis in a variety of cancers. In a murine mammary cancer model, LPS has been found to trigger lung metastasis. However there are very few studies which report the pro-tumor properties of LPS in lung cancer. In this study we investigated the effect of LPS on Fibronectin induced metastatic progression in human lung cancer cells.

Lower concentrations of LPS exhibited a pro-proliferative effect on A549 cells. Using such concentrations of LPS to stimulate A549 cells, expression of Fibronectin mRNA increased in comparison to control. 0.5 -µg of LPS treatment led to the two fold increase in mRNA expression of Fibronectin. Moreover, LPS stimulation augmented the migratory ability of A549 cells as assessed by wound healing assay. This increase in migration of A549 cells was in agreement with the expression pattern of Fibronectin induced by LPS stimulation.

Taken together this study presents a preliminary account of the role of LPS in inducing the expression of Fibronectin and metastatic progression in lung carcinoma.

by Asif Amin | Taseem A. Mokhdomi | Asrar H. Wafai | Raies A. Qadri "Lipopolysaccharide promotes the metastatic Potential of Lung Carcinoma Cells by Upregulating the Expression of Fibronectin"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1 , December 2017,

URL: http://www.ijtsrd.com/papers/ijtsrd7164.pdf

Direct Link - http://www.ijtsrd.com/biological-science/cell-biology/7164/lipopolysaccharide-promotes-the-metastatic-potential-of-lung-carcinoma-cells-by-upregulating-the-expression-of-fibronectin/asif-amin

Cell biology, Open Access Journal

Friday 4 November 2016

Counting of Stomata from Different Types of Leaves

November 04, 2016 0
Counting of Stomata from Different Types of Leaves
Stomata play a vital role in a plant's life. It helps in the process of Photosynthesis and Respiration. The exchange of gases also occurs through stomata. Thus Stomata is very important part of the plant. Few plants belonging to class Dicot and Monocot were collected to study the number of stomata present in their leaves. Leaves from Herbs, Shrubs, Trees and Climbers were collected for the study. These leaves were collected from wide areas from Ahmedabad city. As the stomata are located on the dorsal surface of leaf, the chlorophyll was scrapped from the dorsal surface. The chlorophyll content should be removed to see the stomata clearly.

This chlorophyll content was removed using blade/scalpel and water. The number of stomata was calculated using '˜Micrometer slide'. The scale on '˜Micrometer scale' was 0.01 micrometer. This '˜Micrometer slide' was kept under the microscope and the scale was fixed. After that only the number of stomata was calculated. It was found that '˜Tulsi' had the maximum number of stomata among the dicotyledons whereas '˜Pancreatium sp' had the maximum number of stomata among the Monocotyledons. The stomata were calculated in fresh leaves of collected plants. The number stomata were carried out under '˜Compound Microscope'. The number of stomata is directly related to the enhancement of Physiological process.

By Kirti Suthar | Pooja Yadav | Komal Sutariya | Sheeba Menon" Counting of Stomata from Different Types of Leaves"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-6 , October 2017,

Paper URL: http://www.ijtsrd.com/papers/ijtsrd4717.pdf 

Direct URL: http://www.ijtsrd.com/biological-science/cell-biology/4717/counting-of-stomata-from-different-types-of-leaves/kirti-suthar

Friday 10 June 2016

FtsZ, A Tubulin Homologue Spatiotemporally Regulates the Bacterial Cell Division

June 10, 2016 0
FtsZ, A Tubulin Homologue Spatiotemporally Regulates the Bacterial Cell Division
Most common pattern of Bacterial division is binary fission. Bacterial division occurs by the construction of Z-ring at appropriate time and at a defined site. FtsZ is the major component of Z-ring formation, and the polymerization of FtsZ is regulated in space and time by negative and positive regulators of FtsZ assembly. In the bacterial cell positive and negative regulators help in attaining a critical concentration gradient, such that at mid cell the critical concentration required for Z-ring formation is lowest, thus Z-ring formation takes place at this site only.

By Aijaz Rashid | Shazia Ahad" FtsZ, A Tubulin Homologue Spatiotemporally Regulates the Bacterial Cell Division"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-5 , August 2017,

Paper URL: http://www.ijtsrd.com/papers/ijtsrd2410.pdf 

Direct URL: http://www.ijtsrd.com/biological-science/cell-biology/2410/ftsz-a-tubulin-homologue-spatiotemporally-regulates-the--bacterial-cell-division/aijaz-rashid

Wednesday 10 February 2016

Sperm Motility as a Screening Strategy for the Identification of Microtubule Targeting Drugs

February 10, 2016 0
Sperm Motility as a Screening Strategy for the Identification of Microtubule Targeting Drugs
Microtubule targeting drugs are considered as successful anticancer agents. The hunt for novel microtubules targeting agents is an exciting anticancer research area, as new drugs, may not only show better potency and bioavailability but may also differentially affect the microtubule assembly and thus provide new insights about microtubule dynamics and polymer biology. Cold liable nature of microtubules is bases for tubulin isolation and screening of novel tubulin binding agents. Direct effect of different compounds on microtubule architecture and mitotic index in MCF-7/HeLa cell cultures is also used as a screening strategy.

SRB/MTT assays that measure cell proliferation are also used as preliminary screening strategy to determine the effect of different compounds on cell proliferation for identifying microtubule targeting agents. Keeping in view the complexity of these assay systems, the possibility of sperm motility as a screening strategy for identifying the novel microtubule targeting drugs/agents was explored. So, the effect of known microtubule targeting agents on sperm motility was determined. The results suggest that nocodazole and colchicine does not affect sperm motility, thusimpairment in sperm motility cannot be developed as a general method for screening of microtubule targeting drugs. However, curcumin was identified as a potent compound affecting sperm motility, indicating the possibility that in future curcumin or its derivatives might be used as natural contraceptives.

By Aijaz Rashid" Sperm Motility as a Screening Strategy for the Identification of Microtubule Targeting Drugs"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-4 , June 2017,

Paper URL: http://www.ijtsrd.com/papers/ijtsrd2209.pdf 

Direct URL: http://www.ijtsrd.com/biological-science/cell-biology/2209/sperm-motility-as-a-screening-strategy-for-the-identification-of-microtubule-targeting-drugs/aijaz-rashid

Sunday 7 February 2016

Molecular Mechanism of Microtubules Dynamics and its Precise Regulation Inside Cells

February 07, 2016 0
Molecular Mechanism of Microtubules Dynamics and its Precise Regulation Inside Cells
Microtubules are tubulin polymers that use nucleoside triphosphate (NTP) hydrolysis for polymerization. Microtubules (MTs) are involved in the diverse and dynamic cellular functions like cell shape maintenance, cell division, cell migration, and signalling. Microtubules display dynamic behaviour of Treadmillin and microtubule dynamics these processes are is precisely regulated by microtubule associated proteins. Soluble and polymeric form of tubulin is in equilibrium state that is regulated by microtubule polymerizing and depolymerizing proteins.

By Aijaz Rashid | Shazia Ahad" Molecular Mechanism of Microtubules Dynamics and its Precise Regulation Inside Cells"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-4 , June 2017,

Paper URL: http://www.ijtsrd.com/papers/ijtsrd2214.pdf 

Direct URL: http://www.ijtsrd.com/biological-science/cell-biology/2214/molecular-mechanism-of-microtubules-dynamics-and-its-precise-regulation-inside-cells/aijaz-rashid

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