International Journal of Trend in Scientific Research and Development: Microbiology

International Journal of Trend in Scientific Research and Development

IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas. For any further information, feel free to write us on editor.ijtsrd@gmail.com

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    • Showing posts with label Microbiology. Show all posts
    Showing posts with label Microbiology. Show all posts

    Friday, 15 October 2021

    Isolation and In Vitro Activities of Asplenum Bulbiferum Leaf Extracts on Bacteria Associated with Lung Diseases

    October 15, 2021 0
    Isolation and In Vitro Activities of Asplenum Bulbiferum Leaf Extracts on Bacteria Associated with Lung Diseases
    Lung diseases have been a worldwide health problem, with high endemicity in developing countries. Bronchopneumonia remains the main disease of the lung. Despite advances in modern medicines, there is no effective drug available that stimulate lung function, offer protection to the lung from damage or help to regenerate pulmonary cells destroyed by disseminated bacteria. There is an urgent need for effective drugs to replace supplement those in current use. This study was embacked upon to evaluate the in vitro effect of Asplenium bulbiferum leaf extracts on isolates from patient's sputum. Streptococcus pneumoniae was obtained from human specimen, identified through cultural, morphological and biochemical examination. The antibacterial activity was carried out using agar well diffusion technique. Tube dilution technique was used to determine the minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC using double fold serial dilution at concentrations of 400mg ml to 25mg ml. The photochemical analysis revealed the presence of alkaloids phenolics, tannins, saponins, flavonoids, steroids and glycosides. The ethanolic extract exhibited more activity than the aqueous extract against S. aureus most followed by S. pneumoniae, H. influenza and K. pneumoniae was the least. The study showed that Asplenium bulbiferum possessed antibacterial properties and should serve as alternative therapy for ameliorating lung infections. 
    by Eze, H. C | Ajogwu, T. M | Ozor C. N | Gloria, C. I | Soludo, O. C "Isolation and In-Vitro Activities of Asplenum Bulbiferum Leaf Extracts on Bacteria Associated with Lung Diseases" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 
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    Thursday, 14 October 2021

    The In Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf Extracts on Four Common Bacteria Associated with Lung Diseases

    October 14, 2021 0
    The In Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf Extracts on Four Common Bacteria Associated with Lung Diseases
    Lung diseases have been a worldwide health problem, with high endemicity in developing countries. Bronchopneumonia remains the main disease of the lung. Despite advances in modern medicines, there is no effective drug available that stimulate lung function, offer protection to the lung from damage or help to regenerate pulmonary cells destroyed by disseminated bacteria. There is an urgent need for effective drugs to replace supplement those in current use. This study was embacked upon to evaluate the effect of Asplenium bulbiferum leaf extracts on nosily infected mice with streptococcus pneumoniae isolate of suspension containing 5x106 cells ul. Streptococcus pneumoniae was obtained from human specimen, identified through cultural, morphological and biochemical examination, in addition, polymerase chain reaction PCR technique was performed using universal primers to support the identification process. The antibacterial activity was carried out using agar well diffusion technique. Tube dilution technique was used to determine the minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC using double fold serial dilution at concentrations of 400mg ml to 25mg ml. The effect of the leaf extract was evaluated by treating mus musculus mice WBC count 4.5x103 with sub MIC of A. bulbiferum extracts after nosily infected with S. pneumoniae and appearance of disease septum with 11.3x103 WBC count after 5days from infection. The photochemical analysis revealed the presence of alkaloids phenolics, tannins, saponins, flavonoids, steroids and glycosides. The ethanolic extract exhibited more activity than the aqueous extract against S. aureus most followed by S. pneumoniae, H. influenza and K. pneumoniae was the least. After treating the infected mice with the plant extract and after 6 days from infection, the WBC count reduced to 5.1x103 l which is normal range and the mice were healthy with good physiological behaviour. The infected mice and untreated with extracts, the WBC and other immunological parameters remain high even after 16 days from infection. The study showed that Asplenium bulbiferum possessed antibacterial properties and should serve as alternative therapy for ameliorating lung infections.
    by Eze, H. C | Ajogwu, T. M | Ozor C. N | Gloria, C. I | Soludo, O. C "The In-Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf Extracts on Four Common Bacteria Associated with Lung Diseases" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 
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    Monday, 11 October 2021

    Nutritional and Proximate Assessment of Milk Blends Formulated from Tiger Nut and Soy Beans

    October 11, 2021 0
    Nutritional and Proximate Assessment of Milk Blends Formulated from Tiger Nut and Soy Beans
    The need for vegetable milk as substitute for dairy milk to meet consumers' needs has been on the rise. This is because plant protein has been found to be an alternative to people suffering from lactose intolerance. This study analyzed the nutritive and proximate qualities of milk formulated from Tiger nut and soy beans milk using standard methods. Fresh tiger nut milk and soybean milk were aseptically processed in the Laboratory and blended at varying proportions to obtain seven blends A, B, C, D1, D2, E1 and E2 where A=100 soy milk, B= 100 tiger nut milk, C= 50 tiger nut milk and 50 soy milk, D1= 80 tiger nut milk and 20 soy milk, D2= 20 Tiger nut milk and 80 Soy milk, E1= 60 tiger nut milk and 40 soy milk and E2= 40 Tiger nut milk and 60 Soy milk without addition of spices or chemical preservatives. Results obtained were statistically analyzed with significant different at P=0.5. Organoleptic analysis of the milk blends indicated that all samples were generally acceptable but Sample D1 had the highest acceptability with no significant difference at P 0.05 for mouth feel, taste and colour while there was a significant difference in flavor and overall acceptability at P=0.5 . Nutritive and proximate quality analysis indicated that moisture, carbohydrate, protein and fat were quantitatively the major components of the beverage and the pH fell within the neutral range and is suitable for people suffering from ulcer and other colic problems. Sample D1 had the highest carbohydrate as well as energy value of 9.85 and 86.61kcal g respectively. The total calorific value increased with an increase in carbohydrate, protein and fat contents of the milk blends while total solids increased with decrease in moisture. All samples were highly acceptable but sample D1 received the highest overall acceptability score. An observable trend was that protein increased with an increase in soy milk substitution while fat and carbohydrate increased with an increase in tiger nut milk substitution. Thus, the provision of an enhanced energy drink that is of high nutritional and economical potential. It is suggested that increased Soy milk substitution is required for infants and children while increased Tiger nut milk substitution will be beneficial to Adults. 
    by Okoroafor, G. A. | Umeh, S. O. "Nutritional and Proximate Assessment of Milk Blends Formulated from Tiger Nut and Soy Beans"   

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 
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    Sunday, 3 October 2021

    Phytotoxicity and Microbial Activities of Crude Oil Polluted Pristine Soil during Bioremediation with Saw Dust Amendment from Awka, Nigeria

    October 03, 2021 0
    Phytotoxicity and Microbial Activities of Crude Oil Polluted Pristine Soil during Bioremediation with Saw Dust Amendment from Awka, Nigeria
    The continuous drive to increase the production, distribution and utilization of petroleum and natural gas products to meet the energy demands of the population is usually associated with some environmental pollution problems especially on the lithosphere soil and the physicochemical remediation approaches such as evaporation, dissolution, dispersion with chemical dispersants emulsifiers, and photo oxidation all found to be eco harzardous and non cost ineffective. Microbiological and Physico chemical properties of pristine soil contaminated with crude oil were assessed using standard procedures. The influence of saw dust in the remediation of the polluted soil was evaluated. Phytotoxicity of the polluted soil was assessed using maize and beans seed germination test. Most frequently occurring microbial isolates obtained were identified using molecular typing. Bacteria isolated from the pristine soil were Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Pseudomonas fluorescens, Pseudomonas aeruginosa, Alcaligenes faecalis and Klebsiella pneumoniae. Fungi isolated were Penicillium citrinnum, Aspergillus fumigatus, Aspergillus flavus and Rhizopus stolonifer. The most frequently occurring novel isolates were S. maltophilia, P. sordelli, B. thuringensis, F. solani, C. deightonni, P. citrinnum, and C. bertholletiae. Percentage organic carbon content of the crude oil polluted soil was 21.50 while the nitrogen content was 0.90 with a pH value of 4.69. The Percentage organic carbon of the pristine soil was 0.750 , percentage nitrogen content was 2.71 with a pH value of 5.69. This research showed that phytotoxicity test carried out on the crude oil polluted soil distorted its physico chemical parameters which has consequent toxicity on the soil, thus possibly hampering its fitness for use in crop production. Phytotoxicity test on the saw dust amended soil significantly supported plant growth with a high germination index 1G for both crops maize and beans therefore making the soil fit for use in crop production. 
    by Anagboso, M. O. | Orji, M. U. | Ikele, M. O. "Phytotoxicity and Microbial Activities of Crude-Oil Polluted Pristine Soil during Bioremediation with Saw Dust Amendment from Awka, Nigeria" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 
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    Sunday, 26 September 2021

    Medicinal Effects of Solanum Aethiopicum Leaf Ethanolic Extracts on Renal and Hepatic Function of Wistar Rats

    September 26, 2021 0
    Medicinal Effects of Solanum Aethiopicum Leaf Ethanolic Extracts on Renal and Hepatic Function of Wistar Rats
    The effect of Solanum aethiopicum leaf extracts on, hepatic and renal function was studied. Twenty five wistar albino rats were divided into five groups of five rats each. Apart from the control group, the experimental groups were given compounded feed of ground Solanum aethiopicum leaf and normal pelletized rats feed. Results obtained for hepatic and renal function revealed significance effect p 0.05 on some of the parameters investigated in test rats against those of the control. This study has shown the effect of Solanum aethiopicum leaf extract on hepatic and renal function. 
    by Eze, H. C | Okoli Ik | Ajogwu Tobechukwu Maximilian | Okafor, Onyedika Ifeanyi | Ozor, Chinwendu Njideka "Medicinal Effects of Solanum Aethiopicum Leaf Ethanolic Extracts on Renal and Hepatic Function of Wistar Rats" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 
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    Friday, 17 September 2021

    Microbiological and Physico chemical Examination of Crude Oil Contaminated Soil from Awka Anambra State Nigeria

    September 17, 2021 0
    Microbiological and Physico chemical Examination of Crude Oil Contaminated Soil from Awka Anambra State Nigeria

    Crude oil is a complex mixture of aliphatic and aromatic hydrocarbons that causes a variety of risks when released into agricultural and aquatic environment. This oil can alter population dynamics and disrupt tropic integrations and the structure of natural communities within ecosystems during spills. Microbiological and Physico chemical properties of crude oil and pristine soil samples were assessed using standard procedures. Most frequently occurring microbial isolates obtained were identified using molecular typing. Bacteria isolated from the contaminated soil were Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Pseudomonas fluorescens, Pseudomonas aeruginosa, Alcaligenes faecalis and Klebsiella pneumoniae. Fungi isolated were Penicillium citrinnum, Aspergillus fumigatus, Aspergillus flavus and Rhizopus stolonifer. The most frequently occurring novel isolates were S. maltophilia, P. sordelli, B. thuringensis, F. solani, C. deightonni, P. citrinnum, and C. bertholletiae. Percentage organic carbon content of the crude oil contaminated soil was 21.50 while the nitrogen content was 0.90 with a pH value of 4.69. This research showed that crude oil contamination of agricultural soil distorts its physico chemical parameters which has consequent toxicity on the soil, thus possibly hampering its fitness for use in crop production.

    by Anagboso, M. O. | Orji, M. U. | Ikele, M. O. "Microbiological and Physico-chemical Examination of Crude Oil Contaminated Soil from Awka Anambra State Nigeria" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 

    URL: https://www.ijtsrd.com/papers/ijtsrd46442.pdf 

    Paper URL : https://www.ijtsrd.com/biological-science/microbiology/46442/microbiological-and-physicochemical-examination-of-crude-oil-contaminated-soil-from-awka-anambra-state-nigeria/anagboso-m-o

    callforpaperphysics, physicsjournal
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    Saturday, 4 September 2021

    Evaluation of the Wort Properties of Four Improved Sorghum Varieties Mashed with and Without Commercial Enzyme

    September 04, 2021 0
    Evaluation of the Wort Properties of Four Improved Sorghum Varieties Mashed with and Without Commercial Enzyme

    To determine the wort properties of recently improved sorghum properties, the malt of four sorghum varieties namely Samsorg 46, Samsorg 47, Samsorg 48 and Samsorg 49 were analyzed. The grains were malted and mashed with and without commercial enzyme, using the single decoction mashing technique. The wort derived from the mashing was filtered and boiled, then analyzed. The wort properties looked out for include, specific gravity, Protein estimation using Micro Kjeldahl method, reducing sugar using the DNS method and free a amino nitrogen FAN using method described by Association of Official Analytical Chemists, pH, filterability rate. Generally, there were significant differences across the different varieties of sorghum for, FAN, reducing sugars, cold water extract and hot water extract at both p 0.05 and p 0.01. There was a general increase in specific gravity, reducing sugar, FAN, filterability rate and pH, as the days of germination increased across the varieties, while there was a noticeable decrease in protein content as the days of germination increased in single decoction method of mashing. The varieties gave good results using single decoction method as there were increases in the specific gravity with Samsorg 47 having the highest with 100 malt wort 1.035 and malt enzyme wort 1.036 , reducing sugar with Samsorg 46 having the highest with 100 malt wort 130mg ml and malt enzyme wort 130mg ml , FAN with Samsorg 48 having the highest with 100 malt wort 284.69mg L and Samsorg 49 for malt enzyme wort 351.38mg L and filterability rate with Samsorg 47 having the highest with 100 malt wort 3.00ml min and malt enzyme wort 3.80ml min . The four recently improved sorghum varieties had good wort properties but more works need to be done on then to realize their full potential. 

    by Okoro, N. C. N. | Okonkwo, I. F. | Izundu, M. I "Evaluation of the Wort Properties of Four Improved Sorghum Varieties Mashed with and Without Commercial Enzyme" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 

    URL: https://www.ijtsrd.com/papers/ijtsrd46385.pdf 

    Paper URL : https://www.ijtsrd.com/biological-science/microbiology/46385/evaluation-of-the-wort-properties-of-four-improved-sorghum-varieties-mashed-with-and-without-commercial-enzyme/okoro-n-c-n


    callforpaperarts, artsjournal, peerreviewedjournal 
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