RAPD based Molecular Characterization of Lavatera cachemiriana Cambess

The exploration of genetic diversity is important to properly maintain and exploit germplasm resources and to design better strategically defined approaches in the utilization of collected germplsam so as to safeguard it from extinction. Therefore, current study was undertaken to evaluate genetic diversity of Lavatera cachemiriana collected from different geographical locations.

RAPD based molecular markers were used to evaluate genetic diversity. Total DNA was isolated using CTAB method, molecular marker based polymorphism was carried out using ISSR and RAPD molecular markers, while as genetic analysis was determined in the form of RP, MI, PIC, EMR, Nei-Li's similarity matrix using different tools and techniques.

A total number of 137 DNA fragments were obtained (average- 9.13/primer) which resulted from 15 RAPD primers, among these 33 were found to be monomorphic and rest as polymorphic. The highest polymorphic bands were generated by OPT06 (9) with percentage of polymorphism as 90%. Three RAPD primers have showed number of polymorphic bands between 4-6 i.e. OPB10 (4), OPF03/OPG09 (6) with polymorphic percentage between 44.44%- 75.0%.

PIC value ranged between 0-0.29 with highest value shared by OPT06 (0.23). EMR was found between 0-6.80 with highest value shared by OPG09. Nei's genetic distance (NeiGD) and Shannon's Index (I) were found as 0.119 and 0.075 respectively. EMR was found between 0-6.80 with highest value shared by OPG09. Nei's genetic distance (NeiGD) and Shannon's Index (I) were found as 0.119 and 0.075 respectively.

Two distinct clusters (1 & 2) were obtained which formed a major cluster at 0.98 level of similarity. The cluster 1 belongs to TANG and PAMP samples and showed closer genetic relationship with 0.996 level of similarity. Therefore, the results showed that Lavatera cachemiriana possesses lower but significant levels of polymorphism among collected samples.

by Mudasir A Mir | Amrina Shafi | Insha Zahoor "RAPD based Molecular Characterization of Lavatera cachemiriana Cambess"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1 , December 2017,

URL: http://www.ijtsrd.com/papers/ijtsrd5962.pdf 

Direct URL: http://www.ijtsrd.com/biological-science/biotechnology/5962/rapd-based-molecular-characterization-of-lavatera-cachemiriana-cambess/mudasir-a-mir

UGC Approved Journal, Biotechnology Journal