International Journal of Trend in Scientific Research and Development: Biochemistry

International Journal of Trend in Scientific Research and Development

IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas. For any further information, feel free to write us on editor.ijtsrd@gmail.com

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    • Showing posts with label Biochemistry. Show all posts
    Showing posts with label Biochemistry. Show all posts

    Saturday, 9 October 2021

    Oxidative Stress in Diabetic Obese Patients Attending Selected Tertiary Hospital in Abia State Nigeria

    October 09, 2021 0
    Oxidative Stress in Diabetic Obese Patients Attending Selected Tertiary Hospital in Abia State Nigeria
    Oxidative stress has continued to play major roles in the etiology of many metabolic diseases, diabetes and obesity inclusive. The study on oxidative stress in diabetic obese patients was carried out with patients from two tertiary hospitals in Abia State Nigeria who gave their consent to participate in the study. A total of 120 patients 18 years and above who were stratified into five different age groups were enrolled for the study. Following approved protocols fasting blood samples were collected and a semi structured questionnaire used to collect other data needed for the study. Data was analyzed using T test and SPSS version 20.0 software and result considered significant at p 0.05. The results showed that body mass index BMI were higher in females than males 37.20 vs 35.12 kg m2. The oxidative stress markers or parameters of the experimental groups follow a uniform trend of significant increases in Malondialdehyde MDA , and significant decreases in enzymes Glutathione peroxidases GPx and Superoxide dismutase SOD and vitamins C and E . Also middle aged patients 30 50 years were equally affected with diabetes and more in females than males. Generally increased oxidative stress or impaired antioxidant defense system in diabetic obese condition may contribute to increased metabolic damage. 
    by Egejuru O. Leonard | Akubugwo I. Emmanuel | Chinyere O. Godwin | Ugorji N. Beatrice "Oxidative Stress in Diabetic-Obese Patients Attending Selected Tertiary Hospital in Abia State Nigeria" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 
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    Sunday, 19 September 2021

    Irregularity of Glycogen Synthase Homeostasis Complications Hepatic and Neuro Defects

    September 19, 2021 0
    Irregularity of Glycogen Synthase Homeostasis Complications Hepatic and Neuro Defects
    Glucose homeostasis is strongly regulated to meet the strength needs of major organs and to maintain human health. The liver has a major role in control glucose homeostasis by controlling numerous pathways of glucose metabolism, over eating collection of glycogen union glycogenesis, separate glycogen of glycogenolysis, glucose separate of glycolysis, and under fasting condition amino corrosive into glucose are gluconeogenesis. this metabolic cycle reliant upon admission of food manage eating social huge of our life. Doesnt control influences diabetic, fat, liver sickness and so forth. accumulate facts from hereditary mammal models indicates that the brain, mostly the hypothalamus, has a key major in the homeostatic rule of might and glucose metabolism. Insulin is the activation of glycogen synthase by phosphorylation of the enzyme glucose 6 phosphate ratio. Glycogen is encoded by glycogen synthase, GS1 is expressed in muscle and other tissues, and GS2 is often expressed in the liver, although we have found that GS2 is rarely stored in the brain. We focused liver Glycogen synthase is regulated without delay by glycogen synthase kinase three, AMPK, protein kinase A PKA and casein kinase 2, each protein kinase resulting in a phosphorylated and catalytically inactive glycogen synthase. GS is also regulated by protein phosphatization, which energises glycogen synthase by dephosphorylation. We aimed previous studies on defects of glycogen metabolism.

    by Tamilarasan Shanmugasundaram | Uthirakumar Devaraj | Elumalai Balamurugan "Irregularity of Glycogen Synthase Homeostasis Complications- Hepatic and Neuro Defects" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 
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    Thursday, 26 August 2021

    Comparative Studies of Diabetes in Adult Nigerians Lipid Profile and Antioxidants Vitamins A and C

    August 26, 2021 0
    Comparative Studies of Diabetes in Adult Nigerians Lipid Profile and Antioxidants Vitamins A and C

    The study sought to determine the extent to which the usage of social media in the marketing of agricultural products in South West Nigeria can enhance farmers turnover. It employed the survey research design to collect data with the help of a structured questionnaire to elicit information from respondents selected from six 6 south western states. Research data were analysed using structural equation modelling. The results showed that the use of social media WhatsApp and Facebook in marketing of agricultural products significantly enhances farmers turnover. The managerial implication is that use of Whatsapp and Facebook in the marketing of agricultural products for the enhancement of farmers’ turnover was found to have significant influence on the enhancement in farmers’ turnover from agricultural products. Policy makers in government should provide the enabling environment for the telecommunication companies to enhance their reach by installing their facilities across the length and breadth of the country so that the network coverage will be strong at all times so that the benefits of social media usage will not be constrained. 

    by Egejuru, Leonard O | Akubugwo, Emmanuel I | Ugorji, Beatrice N "Comparative Studies of Diabetes in Adult Nigerians: Lipid Profile and Antioxidants Vitamins (A and C)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, 

    URL: https://www.ijtsrd.com/papers/ijtsrd45021.pdf 

    Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/45021/comparative-studies-of-diabetes-in-adult-nigerians-lipid-profile-and-antioxidants-vitamins-a-and-c/egejuru-leonard-o


    callforpaperinternationaljournal, ugclistofjournals, bestinternationaljournal 
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    Tuesday, 20 July 2021

    Identification of Bioactive Phytochemicals using GC–MS in Leaf Ethanolic Extract of Tragia Involucrata L

    July 20, 2021 0
    Identification of Bioactive Phytochemicals using GC–MS in Leaf Ethanolic Extract of Tragia Involucrata L

    Tragia involucrata is belonging to the family Euphorbiaceae common medicinal herb in Southern India. In the present investigation, air died powdered ethanol extracts of Tragia involucrata leaf sample was analyzed by gas chromatography mass spectrometry GC–MS to identify the important phytochemical constituents. The GC MS analysis has shown the presence of 43 active compounds in the leaf extract. Based on area percentage, the top five major compounds present in the ethanolic extract were Palmitic acid 13.39 , 7Z,10Z,13Z 7,10,13 Hexadecatrienal 11.44 , 3beta,24s Stigmast 5 En 3 One 8.10 , Pipeline 7.37 and Friedelan 3 one 6.01 . The GC MS analysis of selected leaf extract proved that the presence of various bioactive compounds. These bioactive compounds justify, the use of this plant to treat various diseases by traditional practitioners. 


    by Kalaivanan M | A. Saravana Ganthi | M. Padma Sorna Subramanian "Identification of Bioactive Phytochemicals using GC–MS in Leaf Ethanolic Extract of Tragia Involucrata L" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, 

    URL: https://www.ijtsrd.compapers/ijtsrd43651.pdf 

    Paper URL: https://www.ijtsrd.combiological-science/biochemistry/43651/identification-of-bioactive-phytochemicals-using-gc–ms-in-leaf-ethanolic-extract-of-tragia-involucrata-l/kalaivanan-m

    callforpaperpharmacy, pharmacyjournal, internationalpeerreviewedjournal
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    Thursday, 30 July 2020

    GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Panicum Maximum Jacq Leaves

    July 30, 2020 0
    GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Panicum Maximum Jacq Leaves

    hytoconstituents present in the ethanolic extract of Panicum maximum leaves were explored by Gas Chromatography Mass Spectrometry analysis. The compounds were identified by the gas chromatography coupled with the mass spectrometry. The molecular weight and structure of the compounds of Panicum maximum leaves were ascertained by interpretation of the spectrum of GC MS using the database of National Institute of Standard and Technology NIST . GC MS analysis of Panicum maximum leaves revealed the presence of nineteen biological active compounds. The compounds are benzenamine, 4,4 2 4 nitrophenyl ethenylidene bis N,N dimethyl , cyclopropanecarboxylic acid, 1 phenylmethyl , 2,6 bis 1,1 dimethylethyl 4 methylphenyl ester, 4,5 bipyrimidine 2,2 diamine, 4,6 dimethoxy N,N,N,N tetramethyl , 12 Hydroxystearic acid, phenacyl ester, azadirachtin, de E 2 methyl 1 oxo 2 butenyl 1,2 dioxopropyl dihydro , tricyclo 3.3.1.1 3,7 decane, 1,2,2,3,4,4,6,6,8,8,9,9,10,10 tetradecafluoro 5,7 bis trifluoromethyl , spiro cyclohexane 1,3 3H indole 2 carboxy o toluidide, 7 methyl , 1,3,5 triazine 2,4,6 triamine, N,N bis 3 aminophenyl N,N diphenyl ,4 dehydroxy 2,3,3,4,5,6,7 hepta O methylisoorientin, 9,10 anthracenedione, 1,4 bis 2 ethyl 6 methylphenyl amino , l valine, n heptafluorobutyryl , heptadecyl ester, pyrimido 5,4 d pyrimidine, 4,8 di m anisidino 2,6 diethoxy , 2,3,7,7,12,13,17,18 Octaethyl 21H,23H porphine 8 one, 6 fluoro 2 trifluoromethylbenzoic acid, 2 formyl 4,6 dichlorophenyl ester, 2 hydroxyskatole, benzo f cyclopenta a quinolizine 6,7,7a,8,9,10 8H hexacarboxylic acid, 6,7 dihydro , hexamethyl ester, ethyl 2 butyl 3 ethoxycarbonyl methyl amino crotonate , quinoxaline, 2,3 dimethyl , 4H 1 benzopyran 4 one, 5,6 bis acetyloxy 2 3 acetyloxy 4 methoxyphenyl 3,7 dimethoxy It was concluded that the bioactive compounds support the use of Panicum maximum leaves in the treatment of diseases like cancer, diabetes and hypertension. 

    by O. V. Ikpeazu | I. E. Otuokere | K. K. Igwe "GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Panicum Maximum (Jacq) Leaves" 

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-5 , August 2020, 

    URL: https://www.ijtsrd.com/papers/ijtsrd31893.pdf 

    Paper Url :https://www.ijtsrd.com/chemistry/biochemistry/31893/gc–ms-analysis-of-bioactive-compounds-present-in-ethanol-extract-of-panicum-maximum-jacq-leaves/o-v-ikpeazu

    callforpaperbiologicalscience, biologicalsciencejournal
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    Friday, 22 May 2020

    Endothelial Nitric Oxide Synthase T786C Gene Polymorphism TT Genotype is Associated with High Nitric Oxide Levels and Low HOMA IR Levels in Coronary Artery Disease Patients

    May 22, 2020 0
    Endothelial Nitric Oxide Synthase T786C Gene Polymorphism TT Genotype is Associated with High Nitric Oxide Levels and Low HOMA IR Levels in Coronary Artery Disease Patients

    BACKGROUND Data suggest eNOS 786T C gene polymorphism is distinct in specific population group, ethnicity and geographic region and perhaps this genetic variability might produce different results on exposure to various environmental factors 9 14. Besides there is hardly any data available in Indian population. Sofurther research is needed to explore the complex interaction between environmental factors and eNOS 786T C gene polymorphism in susceptibility to insulin resistance in patients with CAD in Indian population.AIM This study aimed to study the association of Insulin Resistance, Nitric Oxide and intergenotypic variation of endothelial Nitric Oxide Synthase T786C gene polymorphism among coronary artery disease subjects. METHODOLOGY This study consisted of 60 adult patients of with documented CAD and 60 age and sex matched healthy subjects as controls.Fasting Serum Insulin was measured by ELISA,fasting Serum nitric oxide by modified Griess reaction and fasting plasma glucose on fully automated chemistry analyzer Hitachi 902 HOMA IR was calculated mathematically by using formula given by Matthew et al. The eNOS gene loci was amplified by using PCR and by RFLP. A p value 0.05 was considered significant. Statistical analysis was performed with the help of SPSS version.. RESULTS Intergenotypic levels of nitric oxide were significantly lower in TC genotype than TT both in study as well as control groups. Also, TC genotype showed significantly higher HOMA IR levels as compared to TT genotype in both study as well as control group. Moreover, CC genotype could not be found in both cases and control groups..Thus, TT genotype may have a protective role in development of insulin resistance and coronary artery disease as seen by high Nitric Oxide levels and low HOMA IR levels in TT genotype than TC genotypes in both case as well as control groups.CONCLUSIONS Endothelial nitric oxide synthase t786c gene polymorphism tt genotype is associated with high nitric oxide levels and low homa ir levels in coronary artery disease patients. 


    BY Namrata Bhutani | Deepak Tangadi | Neha Bhutani "Endothelial Nitric Oxide Synthase (T786C) Gene Polymorphism TT Genotype is Associated with High Nitric Oxide Levels and Low HOMA-IR Levels in Coronary Artery Disease Patients"

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020,

    URL: https://www.ijtsrd.com/papers/ijtsrd31317.pdf

    Paper Url :https://www.ijtsrd.com/biological-science/biochemistry/31317/endothelial-nitric-oxide-synthase-t786c-gene-polymorphism-tt-genotype-is-associated-with-high-nitric-oxide-levels-and-low-homair-levels-in-coronary-artery-disease-patients/namrata-bhutani

    openaccessjournalofengineering, engineeringjournal, paperpublicationforengineering
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    Thursday, 21 May 2020

    Role of Pre Therapeutic Expression of Human Organic Cation Transporter1 SLC22A1 Gene in Predicting Clinical Outcome in Imatinib Treated CP CML Patients

    May 21, 2020 0
    Role of Pre Therapeutic Expression of Human Organic Cation Transporter1 SLC22A1 Gene in Predicting Clinical Outcome in Imatinib Treated CP CML Patients

    Background Human organic cation transporter1 hOCT1, SLC22A1 , an influx transporter,is responsible for the uptake of Imatinib into chronic myelod leukemia CML cells. Some patients fail to achieve optimal molecular response to Imatinib, defined as major molecular response MMR i.e.BCR ABL 1 = 0.1 within 12 months of therapy. Pretherapeutic expression of hOCT1 may be beneficial in predicting the response to imatinib in CML patients. Methodology 30 newly diagnosed BCR ABL positive CML patients in chronic phaseand30 healthy control subjects, all ethnic Indians ,were recruited in the study. hOCT1 gene expression in PBMCs was quantified by SYBR Green based qRT PCR, using the 2 DDCt method. After initiation of imatinib therapy, hematological response was monitored at regular intervals, and molecular response BCR ABL1 ABL1 ratio assessed after 6 or 12 months. Results and discussion The cases were divided into two groups, high expression n=15 and low expression n=15 groups, based on the median value of fold change in hOCT1 gene expression. Median =5.6 . 11 73.33 patients with low expression achieved CHR by the end of 3 months, whereas 4 26.66 did not. On the contrary, all 15 patients 100 with high hOCT1 gene expression achieved CHR by the end of 3 months. p=0.10 .It was also observed that the mean THR time to CHR in low expression group was higher than in high expression group. p=0.046 It was seen that while all 15 patients with high expression had an optimal response , only 13.33 n=2 patients with low expression had it. In the low expression group, 40 patients had treatment failure n=6 while remaining 23.33 n=7 were categorized as warning. p=0.000 ELN 2013 guidelines Conclusions Hence it was concluded that high expression of hOCT1 gene leads to early achievement of CHR. In case of molecular response, it was observed that high expression of hOCT1 gene was significantly associated with achievement of on optimal response to imatinib. These findings emphasize that knowledge of pretherapeutic level of hOCT1 could be a useful marker to predict imatinib therapy outcome in CML patients, and the prospects of personalized therapy in such patients. 


    BY Namrata Bhutani | Deepika Arora | Neha Bhutani "Role of Pre-Therapeutic Expression of Human Organic Cation Transporter1 (SLC22A1) Gene in Predicting Clinical Outcome in Imatinib Treated CP-CML Patients"

    Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020,

    URL: https://www.ijtsrd.com/papers/ijtsrd31316.pdf

    Paper Url :https://www.ijtsrd.com/biological-science/biochemistry/31316/role-of-pretherapeutic-expression-of-human-organic-cation-transporter1-slc22a1-gene-in-predicting-clinical-outcome-in-imatinib-treated-cpcml-patients/namrata-bhutani

    internationaljournalsinengineering, callforpaperengineering, ugcapprovedengineeringjournal 
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