International Journal of Trend in Scientific Research and Development

The brain is a delicate organ, and evolution built very efficient ways to protect it. Unfortunately, the same mechanisms that protect it against intrusive chemicals can also frustrate therapeutic interventions. Many existing pharmaceuticals are rendered ineffective in the treatment of cerebral diseases due to our inability to effectively deliver and sustain them within the brain. General methods that can enhance drug delivery to the brain are, therefore, of great interest. Despite aggressive research, patients suffering from fatal and or debilitating central nervous system CNS diseases, such as brain tumors, HIV encephalopathy, epilepsy, cerebrovascular diseases and neurodegenerative disorders, far outnumber those dying of all types of systemic cancer or heart disease. The clinical failure of much potentially effective therapeutics is often not due to a lack of drug potency but rather to shortcomings in the method by which the drug is delivered. Treating CNS diseases is particularly challenging because a variety of formidable obstacles often impede drug delivery to the brain and spinal cord. By localizing drugs at their desired site of action one can reduce toxicity and increase treatment efficiency. In response to the insufficiency in conventional delivery mechanisms, aggressive research efforts have recently focused on the development of new strategies to more effectively deliver drug molecules to the CNS. This review intends to detail the recent advances in the field of brain targeting, rational drug design approach and drug delivery to CNS. To illustrate the complexity of the problems that have to be overcome for successful brain targeting, a brief intercellular characterization of the blood-brain barrier BBB is also included. 

by Parth Nikam | Rishikesh Bachhav "A Comprehensive Review on Drug Delivery Approach to Central Nervous System" 

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 

URL: https://www.ijtsrd.com/papers/ijtsrd46375.pdf 

Paper URL : https://www.ijtsrd.com/pharmacy/analytical-chemistry/46375/a-comprehensive-review-on-drug-delivery-approach-to-central-nervous-system/parth-nikam

callforpaperpharmacy, pharmacyjournal, internationalpeerreviewedjournal

HPLC is a chromatographic technique employed in active compound chemistry and biochemistry to separate a mixture and substances with the goal of identifying, measuring, and purifying the different components of the mixture. Its a much better variety of column and traditional chromatography. The objective of the research work is to develop and validate a simple and accurate reverse phase chromatographic method to estimate amount of drug in dosage form. The developed method successfully can be applied to estimate the amount of Dapagliflozin in tablet dosage form. After oral administration of dapagliflozin, the maximum plasma concentration Concentration max under two hours. High performance liquid chromatographic system was alleviated according to the chromatographic settings. After attaining the steady base line, to verify the system suitability, a single 40 µg ml of standard solution proportional to 100 test concentration of dapagliflozin was injected into the HPLC system. The gradient mobile phase flow rate programming assisted in optimising the lengthy run duration and resolution of sample analysis, making the approach more cost effective and quick. Validation of the developed and optimized HPLC method was carried out according to ICH guidelines with respect to parameters such as linearity, specificity, precision and accuracy. 

by Junaid Ahmed | Himanchal Sharma | Shiva Teotia "Method Development and Validation for Estimation of Oral Hypoglycaemic Drug Dapagliflozinina Tablet Dosage form by the Employment of Rp-HPLC" 

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 

URL: https://www.ijtsrd.com/papers/ijtsrd46395.pdf 

Paper URL : https://www.ijtsrd.com/pharmacy/analytical-chemistry/46395/method-development-and-validation-for-estimation-of-oral-hypoglycaemic-drug-dapagliflozinina-tablet-dosage-form-by-the-employment-of-rphplc/junaid-ahmed

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High performance liquid chromatography is most powerful tools in analytical chemistry which assessing drug product stability. It is most accurate method for determining the qualitative and quantitative analysis of drug product. Forced degradation plays an important role in development of stability indicating analytical methodology. Stability indicating HPLC methods are used to separate various drug related impurities that are formed during the synthesis or manufacture of drug product. This article discusses the strategies and issues regarding the development of stability indicating HPLC system for drug substance. Forced degradation studies establish degradation pathways of drug substances and drug products. Forced degradation elucidate the possible degradation pathway of the drug substance or the active pharmaceutical ingredient in the drug product. At every stage of drug development practical recommendations are provided which will help to avoid failure. 

by Rushikesh S Mulay | Rishikesh S Bachhav "Stability Indicating HPLC Method Development - A Review" 

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 

URL: https://www.ijtsrd.com/papers/ijtsrd46342.pdf 

Paper URL : https://www.ijtsrd.com/pharmacy/analytical-chemistry/46342/stability-indicating-hplc-method-development--a-review/rushikesh-s-mulay

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Everolimus is semisynthetic derivative of sirolimus, which is isolated from Streptomyces hygroscopicus. A novel reversed phase high performnce liquid chromatography HPLC method for determination of the everolimus in the presence of degradation product or pharmaceutical excipients. Seperation was carried out using Cosmosil C18 250mm x 4. 6ID , column having particle size 5 micron using acetonitrile and methanol mixture, and pH adjusted to 3, a flow rate of 1. 0 mL min, and ultraviolet detection at 285nm. A retention time nearly 3. 806 min was observed. The calibration curve for everolimus was linear from range of 5 25 µg mL with limit of detection and limit of quantitation of 0. 0817 and 0. 2478 µg mL, respectively. Analytical validation parameters such as selectivity, specificity, linearity, accuracy and precision were evaluated and relative standard deviation value for all the key parameters were less than 2. 0 . The stability indicating method was developed by exposing the drug to stress conditions of acid and base hydrolysis, oxidation, photodegradation, and thermal degradation the obtained degraded products were successfully seperated from the APIs. This method was validated in acceptance with ICH guidelines and results within the acceptance criteria. 

by Rushikesh Mulay | Rishikesh Bachhav "Stability Indicating RP-HPLC Method Development and Validation of Everolimus in Bulk and Pharmaceutical Dosage Form" 

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 

URL: https://www.ijtsrd.com/papers/ijtsrd46341.pdf 

Paper URL : https://www.ijtsrd.com/pharmacy/analytical-chemistry/46341/stability-indicating-rphplc-method-development-and-validation-of-everolimus-in-bulk-and-pharmaceutical-dosage-form/rushikesh-mulay

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Cancer is the most prevalent disease not only in the United States but worldwide. The most representative polyphenol component extracted from the rhizomes of Curcuma long a known as turmeric is curcumin. The therapeutic benefits of curcumin have been demonstrated in multiple chronic diseases inflammation, arthritis, metabolic syndrome, liver disease, obesity, neurodegenerative diseases and, above all, in several cancers. Chemotherapy is a major form of treatment modality for various human diseases and disorders in both developing and developed countries. This intervention has been associated with a number of side effects and poor compliance. Therefore, in recent years, a significant effort has been put forward for finding a better treatment modality that uses natural compounds or extracts. Among many naturally occurring polyphenol compounds, curcumin is a highly safe yellow pigment molecule, widely used as a food coloring agent, and can be used to treat various pathological conditions. 

by Rushikesh Mulay | Rishikesh Bachhav "Curcumin Based Nanotherapy of Cancer - A Review" 

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, 

URL: https://www.ijtsrd.com/papers/ijtsrd46376.pdf 

Paper URL : https://www.ijtsrd.com/pharmacy/analytical-chemistry/46376/curcumin-based-nanotherapy-of-cancer--a-review/rushikesh-mulay

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A simple, sensitive, rapid and reproducible HPLC method has been developed and validated for Calibration determination of Valsartan in bulk and in pharmaceutical formulation by applying Quality intentionally. For development of HPLC method for Valsartan various trials are performed by using Design Expert software by applying 3 level factorial designs. Quantitative method development by optimization from trials intentionally Expert software. The Optimized method Desirabilityis0.998 for Mobile Phase ACN Phosphate Buffer 65 35 in3.5 pH at maximum Wavelength 274nm at column oven temperature 40°C. The flow of mobile phase was adjusted 1.0 ml min. and therefore the injection volume 10 µl. Optimised Standard curve showed a parametric statistic is 0.998. Retention time was found to be 4.6 min. the half of recovery was found to be within the bounds of the acceptance criteria with average recovery of 99.4 you take care of Valsartan. The tactic was validated as per ICH guidelines. The precision and repeatability results showed RSD but 2 . The developed method was successfully validated in consistent with ICH guidelines. Hence, these methods are often conveniently adopted for the routine analysis in internal control laboratories.